Immobilization of Fibronectin-Loaded Polyelectrolyte Nanoparticles on Cardiovascular Material Surface to Improve the Biocompatibility

Shihui Liu,Chunzhi Tang,Qingwei Huo,Lin Wang,Rongrong Tao,Nenggui Xu,Tao Gong,Changjiang Pan,Tao Liu,Youdong Hu

doi:10.1155/2019/5478369

Abstract

Vascular stent interventional therapy is the main method for clinical treatment of coronary artery diseases. However, due to the insufficient biocompatibility of cardiovascular materials, the implantation of stents often leads to serious adverse cardiac events. Surface biofunctional modification to improve the biocompatibility of vascular stents has been the focus of current research. In this study, based on the structure and function of extracellular matrix on vascular injury healing, a novel fibronectin-loaded poly-l-lysine/heparin nanoparticles was constructed for stent surface modification. In vitro blood compatibility evaluation results showed that the nanoparticles-modified surface could effectively reduce platelet adhesion and activation. In vitro cellular compatibility evaluation results indicated that the nanocoating may provide adequate efficacy in promoting the adhesion and proliferation of endothelial cells and thereby accelerate endothelialization. This study provides a new approach for the surface biological function modification of vascular stents.

Highlights

Cardiovascular diseases (CVDs), such as coronary artery diseases (CADs), continue to be the leading cause of death globally [1]
With the in-depth exploration of the mechanism of pathological response induced by material implantation, it is widely accepted that the rapid endothelialization on the surface of cardiovascular materials after implantation is an ideal way to reduce the risk of postoperative complications
Extracellular matrix components can promote cell growth and lead to platelet adhesion and activation and cause thrombosis [11, 12], BioMed Research International which greatly limits the application of extracellular matrix coating in the field of cardiovascular materials. erefore, it is necessary to find a way to construct the biological coating that may provide adequate properties to inhibit the function of protein molecules in promoting coagulation reaction without affecting the positive efficacy on endothelium regeneration

Summary

Introduction

Cardiovascular diseases (CVDs), such as coronary artery diseases (CADs), continue to be the leading cause of death globally [1]. Because of the important role of extracellular matrix components in cell growth, extracellular matrix proteins are commonly used in surface modification of biomaterials to promote the growth behavior of vascular cells. Matrix proteins and some adhesive proteins, such as fibronectin (Fn) [5, 6], laminin (Ln) [7, 8], and collagen (Col) [9, 10], may bind to the specific receptors that are expressed on endothelial cells (ECs) and initiate a variety of intracellular pathways so as to promote a series of biological behaviors, such as cell adhesion, migration, growth, and proliferation. Extracellular matrix components can promote cell growth and lead to platelet adhesion and activation and cause thrombosis [11, 12], BioMed Research International which greatly limits the application of extracellular matrix coating in the field of cardiovascular materials. Extracellular matrix components can promote cell growth and lead to platelet adhesion and activation and cause thrombosis [11, 12], BioMed Research International which greatly limits the application of extracellular matrix coating in the field of cardiovascular materials. erefore, it is necessary to find a way to construct the biological coating that may provide adequate properties to inhibit the function of protein molecules in promoting coagulation reaction without affecting the positive efficacy on endothelium regeneration

Methods

Results

Conclusion