Abstract
Various enzymes such as glucose oxidase, β- d-glucosidase, acid phosphatase. tyrosinase and laccases were immobilized on clays and soils activated with 3-aminopropyltriethoxysilane and glutaraldehyde. After immobilization the enzymes retained a large amount of their original activities. The retention of laccase activity increased with the increase of clay content in soils, whereas the activity of β- d-glucosidase, tyrosinase and acid phosphatase decreased. The immobilized enzymes showed varying degrees of resistance to proteolysis and storage at high temperatures. After addition to soil suspensions, soluble laccase and glucose oxidase were rapidly inactivated (100 and 85% loss of activity in 15 days for laccase and glucose oxidase, respectively) whereas after immobilization, these enzymes were extraordinarily stable (12 and 25% loss of activity in 15 days for laccase and glucose oxidase, respectively). The possibility of incorporating the stabilized enzymes into soil for the improvement of numerous desired biochemical processes is discussed.
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