Abstract
Immobilization of enzymes by a double entrapping method was investigated, α-Amylase was trapped by radiation-induced low temperature polymerization of 2-hydroxyethyl methacrylate after being absorbed by various inorganic additives. Drierite showed the most remarkable effect by increased enzymatic activity and decreased activity loss in batch enzyme reactions. Double entrapping of cellulase was tried by preparing an entangled mixture of enzyme and various natural polymers as additives and then polymerizing with the monomeric system at low temperatures. The composite, including enzyme-additive mixture prepared by coprecipitating and coevaporating methods, showed a considerable enhancing effect on enzymatic activity and durability in batch enzyme reactions.
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