Abstract
This current work describes the electrochemical biosensor using immobilization of enzyme on nanomaterial modified glassy carbon electrode. Glucose oxidase, Horseradish peroxidase (HRP), Catalase (CAT), and alcohol dehydrogenase (ADH) enzymes were immobized on modified glassy carbon electrode for biosensor fabrication. Gelatin-Multiwalled carbon nanotube (GCNT), Bismuth oxide nanoparticles-MWCNT composite, didodecyldimethylammonium bromide (DDAB) present on nafion dispersed multiwalled carbon nanotubes (MWCNTs-NF) and biocomposite of toluidine blue O with adsorbed alcohol dehydrogenase (ADH) modified on glassy carbon electrode were prepared for enzyme immobilization. The surface morphology of enzyme immobilized modified electrode were characterized by Scanning electron microscopy. GOx/GCNT, NF/HRP/Bi2O3-MWCNT, MWCNTs-NF-(DDAB/CAT) and ADH/TBO/NF films exhibited a wide linear response from 6.30 to 20.09 mM glucose, 8.34-28.88 mM of (H2O2), 0.5 to 1.2 mM of H2O2 and 283-856 mM ethanol respectively. These films showed sensitivity of 2.47 µA mM-1 cm-2 , 26.54 µA mM-1 cm-2, 101.74 µA mM-1 cm-2 , 7.91 µA M-1 cm-2 respectively. The developed enzyme immobilization on nanomaterial modified glassy carbon electrode has a good operational stability and specificity towards the determination of analytes.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
