Immobilization of double-stranded DNA onto glass beads by psolaren

Abstract

We demonstrated the immobilization of double-stranded DNA onto the glass beads by psoralen, one of the DNA-intercalators in nature. As a result, DNA-immobilized glass beads (DNA-P-beads) were prepared by the intercalation of psoralen, which was immobilized onto the glass surface, onto the double-stranded DNA. These DNA-P-beads formed covalent bondings between psoralen and the nucleic acid base by 365nm UV irradiation. The amount of immobilized-DNA was 0.24mg per gram of glass beads. These DNA-P-beads were stable in water, and the DNA on the bead surface maintained its double-stranded structure. These DNA-P-beads selectively removed the planar-structure containing harmful compounds, such as dioxin and polychlorinated biphenyl (PCB) derivatives, from an aqueous multi-component solution. Additionally, a DNA-P-bead column effectively removed harmful compounds. Furthermore, the DNA-P-bead column could be reused by the addition of common organic solvents, such as ethanol.