Abstract
In this study, chitosanase cloning from Streptomyces albolongus was fermented and purified by a Ni-NTA column. Fe3O4-SiO2 magnetite nanoparticles (MNPs) were synthesized by the co-precipitation method coating with silica via a sol-gel reaction and were then amino functioned by treating with 3-aminopropyltriethoxysilane. Chitosanases were immobilized onto the surface of MNPs by covalent bonding (MNPs@chitosanase). Transmission electron microscopy (TEM), Fourier transform infrared spectrometer (FT–IR), and magnetic measurements were used to illustrate the MNPs and immobilized chitosanase. The optimal conditions of immobilization were studied. The thermal, pH, and stabilities of immobilized chitosanase were tested and the results showed that the stabilities were significantly enhanced compared with free chitosanase. After being recycled 10 times, the residual activity of the immobilized chitosanase was 43.7% of the initial activity.
Highlights
Chitosan oligosaccharides (COS) are depolymerized derivatives of chitosan with degrees of polymerization less than 20, which consist of D-glucosamine via β-1,4-glycoside linkages [1].Chitosan-oligosaccharides have some physiological activities, including antimicrobial activities [2,3,4], immuno-enhancing effects [5], and anti-tumour activities [6]
The magnetic behaviours were confirmed by the vibrating sample magnetometer, which were typical plot of magnetization against applied magnetic field at room temperature, and crossed the zero point, inductive of the superparamagnetic properties
The results suggested that Magnetic nanoparticles (MNPs) protected the enzyme from alkaline and acid to enhance the pH stability [26], which could widen its application in the industry
Summary
Chitosan oligosaccharides (COS) are depolymerized derivatives of chitosan with degrees of polymerization less than 20, which consist of D-glucosamine via β-1,4-glycoside linkages [1]. Magnetic nanoparticles (MNPs) haveof been proved to be efficient materials for enzyme cost-effectiveness are the key properties immobilization techniques for industrial application [17]. Immobilization due to their nanoscale size and nonchemical separation from the reaction mixture by Magnetic nanoparticles (MNPs) have been proved to be efficient materials for enzyme immobilization applying a magnetic field [18]. Among carriers for enzyme immobilization, amino-functionalized magnetic nanoparticles glutaraldehyde, carbodiimide activity, and active epoxy groups [20,21,22]. MNPs immobilization, amino-functionalized magnetic nanoparticles were proved toprevious be simple, cost-effective were developed as a tool to immobilize lipase [15], and co-immobilize β-agarase, and and with high performance [23]. The stability, recycling, and catalytic efficient of immobilized enzymes were investigated
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