Abstract
Immobilization of the hydrocarbonrich microalga Botryococcus braunii in calcium alginate beads results in a large increase in chlorophyll content and chlorophyll photosynthetic activity, relative to free cells, at any stage of standard batch cultures. Immobilization exerts a protective influence on ageing cultures both under standard and air lift conditions. Decreases in chlorophyll content and photosynthetic activity are delayed and slowed down; the organization of protein-chlorophyll complexes is stabilized. These positive effects are related to protection of entrapped cells against photoinhibition owing to gel screening and self-shadowing.
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