Immobilization of Bacillus sp. SLII-1 on chitosan-alginate hybrid material for promising feedstock supplement

Abstract

The increase in poultry meat production led to an increase in feed demand. On the other hand, poultry industry produces feather waste which composes 5-7 wt% of the total body weight. Feed diversification by utilizing feather waste can be an alternative to overcome the problem of the high price of feed and to reduce environmental waste. The use of feather as a feed has a disadvantage because of the low digestibility of feather waste. The strategy to increase the digestibility of feather waste is to deliver keratinolytic bacteria to poultry intestine using immobilization technique. The purpose of this study was to determine the sodium alginate and chitosan concentration of immobilant with the highest number of released cell in poultry digestive system simulation. Bacillus sp. SLII-I as keratinolytic bacteria was able to increase soluble protein in feather meal liquid medium by 117.69% (wt/v). The composition of sodium alginate (2% w/v) and chitosan (0,8% w/v) was the most effective in delivering bacteria to poultry intestine as much as 2.54x107 CFU per gram bead in digestive system simulation.