Abstract
PurposeThe aim of this study is to develop a new coating for wound dressings that is comprised of antimicrobial silver (Ag) and antioxidant flavonoid quercetin (Q).MethodsDip-coating was used to apply the coating on cotton gauge as a model dressing. Ag was immobilised using polydopamine as a priming and catalytic layer followed by coating of quercetin that was incorporated in a functionalized polydimethylsiloxane. The coating was investigated using scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDX) and release assay. The antimicrobial activity of quercetin and Ag was tested against Staphylococcus aureus (S. aureus). A surgical wound model on mice was used to evaluate the effects of the coated dressing on wound healing rates and tissue histology.ResultsAg and quercetin showed enhanced antimicrobial activity against S. aureus when used in combination. Ag and quercetin were successfully immobilized onto the fibre of the dressing using the dip-coating process. The coating released Ag and quercetin over 8 days and showed strong antioxidant activity. In the wound healing model, complete wound closure was achieved in 12 days in the group receiving coated dressing and was associated with an enhancement in tissue remodelling and neo-angiogenesis and the reduction in tissue inflammation.ConclusionThese new antimicrobial-antioxidant coatings may be promising in the development of advanced wound care therapies.
Highlights
Bacterial infection is among the most common and severe complications in the management of wound healing
NaHCO3, Na2CO3, AgNO3, dopamine hydrochloride (DA), quercetin, 2,2-diphenyl-1-picrylhydrazyl (DPPH), isopropanol, methoxyl, amine-functionalised silicone, haematoxylin and eosin (H&E), and Masson’s trichrome (MT) staining kit were purchased from Sigma-Aldrich, USA
energy-dispersive X-ray spectroscopy (EDX) analysis and backscatter electron (BSE) imaging were used to confirm the presence of Ag submicro-/nano-particles on the dressing
Summary
Bacterial infection is among the most common and severe complications in the management of wound healing. The use of antimicrobial wound dressings in infection control has emerged as a promising strategy. The regulation of oxidative stress is another important factor in successful wound healing; it has often been overlooked in the development of wound dressings. The inflammation phase plays an important role in wound healing, dictating the rate and the quality of tissue repair. During this period, an elevated level of reactive oxygen species (ROS) from inflammation may, lead to excessive oxidative stress, induce apoptosis, and impair the healing process.[1,2,3]
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