Abstract

In this Research Study immobilization of Alcohol Dehydrogenase enzyme on glass beads was done. k-carrageenan is a natural polysaccharide like cellulose obtained from seaweeds. ADH was immobilized by Covalent coupling. Then for further study, various parameters like Thermostability , pH study, storage stability and Reusability were optimized and carried out the comparison studies. Furthermore, FTIR analysis was done to prove that the enzyme was immobilized on the support bead. And the application was done with 3 different fruit juices. i.e. sugarcane juice, pineapple juice, and watermelon juice. In addition, this study introduces a new achievement of the eco-friendly effect of immobilized enzyme.

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