Abstract
Spores of fungal strain Humicola lutea 120-5 were immobilized with photo-crosslinkable prepolymer. The entrapped spores were allowed to germinate and develop in situ. The immobilized growing mycelium thus obtained was reused in batch mode for extracellular acid proteinase production. The influence of prepolymer concentration, initial inoculum gel volume, spore content, and duration of the growth cycle on the enzyme activity and mycelium growth were studied. The best yield of the acid proteinase was reached with 15 ml initial gel volume, 20% prepolymer concentration, 10 6 spores per ml gel and 56 h duration of one cycle. The optimization of these factors, which affect the stability and productivity of the immobilized system, resulted in the increase of the enzyme activity (up to 180% compared with free cells), decrease of concentration of outgrowing cells in the medium, and the realization of semicontinuous cultivation. The productive life was nearly 10–12-times longer, and the cumulative enzyme activity was 18-times higher in comparison with those of free culture. Therefore, the immobilized growing Humicola lutea 120−5 in the photo-crosslinked polymer could be used for a long-term production of an acid proteinase.
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