Abstract
We have developed a novel immobilization medium by soaking enzyme-containing calcium alginate beads in a solution of tetramethoxy-ortho-silicate (TMOS) in hexane. Water-soluble alkyl siloxanes, resulting from the partial hydrolysis of TMOS at the hexane-water interface permeated the alginate gel, polymerized and formed a colloid within the beads. Silicate-alginate beads were robust, and did not deform under pressure. The composite gel demonstrated the excellent protein retention and thermal stabilization properties typical of silicate sol-gel-immobilization, as well as protection against inactiva-tion by concentrated urea solutions. Immobilized β-glucosidase had complete operational stability at ambient temperature for at least several months.
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