Abstract
An immobilization engineering approach using bioinformatics and experimental design tools was applied to improve the sol–gel enzyme entrapment methodology.
Highlights
Biocatalysis, as an efficient and green tool for modern organic synthesis, is becoming increasingly important due to its high activity, high chemo, regio, and/or stereoselectivity under mild reaction conditions, and limited by-product formation.[1]
The nature of precursors can influence the morphology, permeability, and hydrophobic/hydrophilic character of the resulting sol–gel network, and on the other hand – as recent studies have shown – organosilanes can exert an imprinting effect on the enzyme altering its catalytic properties.[25,26]. Finding these organosilanes as components for experimental design which can exert a significant imprinting effect has become feasible by computational methods
The synergistic application of bioinformatics and experimental design tools permitted the rapid optimization of entrapment systems providing biocatalysts with improved properties (Fig. 1)
Summary
Biocatalysis, as an efficient and green tool for modern organic synthesis, is becoming increasingly important due to its high activity, high chemo-, regio-, and/or stereoselectivity under mild reaction conditions, and limited by-product formation.[1] Biocatalysts permit the transformation of multifunctional molecules with functional group selectivity, generally obviating functional group activation, protection, and deprotection steps required in traditional organic syntheses.[2] The advantages of reducing the number of synthetic steps – such as reduction of waste and hazards, improvement of the overall yield and cutting of costs – are obvious In spite of their benefits, the use of chemo/enzymatic routes is limited because the relevant enzymes are often commercially not available, and the development of robust biocatalysts suitable for industrial processes is slow and expensive.[3]. Both natural and engineered enzymes are “naked” proteins which might be further decorated to endow them with additional properties to extend their scope of applications in various reactions in a green manner
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