Abstract
Enhanced Green Fluorescent Protein has been covalently immobilized on thin perforated Al films with short-order nanohole arrays using (3-Glycidyloxypropyl)trimethoxysilane and native aluminum oxide layer. The nanoholes’ diameter varied in range 60–120 nm, allowing to tune the surface plasmon resonance frequency. The fluorescence intensity enhancement up to factor 2.75 for the nanohole arrays with the largest overlap between EGFP fluorescence band and surface plasmon resonance.
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