Abstract
This communication reports the application of layer-structured MoS2 as matrix for enzyme immobilization and biosensor construction. MoS2 nanosheets were prepared using ultrasonic exfoliation in solvent mixture. Data from UV–vis spectra, scanning electron microscopy and transmission electron microscopy demonstrate that the as-prepared MoS2 nanomaterial has ordered layer-structure. The negative charges carried by the MoS2 nanosheets are then used to immobilize horseradish peroxidase (HRP) via the electrostatic attraction, forming HRP–MoS2 hybrid. FTIR spectra indicate that the immobilized HRP retains its native structure. The HRP–MoS2 hybrid modified glassy carbon electrode shows good electrocatalytic activity toward H2O2 reduction. This work suggests that MoS2 nanosheets are a potential candidate for efficient enzyme immobilization and promising in biosensing and biocatalytic applications.
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