Abstract
The immobilisation of antibody on silicon membranes containing arrays of micropores was studied. The membranes are modified by the deposition of aminopropyl triethoxysilane to functionalise the surface with amino groups. The surfaces are subsequently treated with 1,4-phenyldiisothiocyanate and Cy5-labelled anti-Human IgG. Fluorescence microscopy demonstrated the immobilisation of the labelled-antibody across the silicon surfaces but not within the micropores. Electrochemistry at liquid/liquid interfaces located within the micropores demonstrated that the pores remained open to ion transfer processes, as shown by the transfer of tetraethylammonium across the micro-interfaces between immiscible electrolyte solutions. These modified membranes will find applications in bioanalytical methods and sample preparation for the analysis of complex biological samples.
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