Immediate intervention effect of dielectric barrier discharge on acute inflammation in rabbit’s ear wound

Chengbiao Ding,Yun Zhou,Tao Jin,Linfei Feng,Pengpeng Huang,Yi Liu,Yuanyuan He,Zhengwei Wu

doi:10.1063/1.5139953

Abstract

Acute inflammation is an anti-injury stress response which is beneficial to the human body. However, if the duration of acute inflammation is prolonged in specific people, such as critically ill patients and people with low resistance, it will worsen the disease and increase complication rates. Most medical researchers are committed to finding an effective method to shorten the duration of acute inflammation. The purpose of our work is to shorten the duration of acute inflammation in a rabbit’s ear wound by dielectric barrier discharge (DBD) low temperature plasma. The distribution and quantity of inflammatory cells in the rabbit’s ear wound treated by plasma were studied and calculated by using a high power optical microscope and Image J software. The reactive species of plasma were characterized by optical emission spectroscopy. The results showed that the number of inflammatory cells decreased from 2312.17 ± 242.52 to 880.17 ± 89.08 after plasma treatment for 3 min. In addition, plasma has a coagulation effect on the rabbit’s ear wound. Our results indicated that DBD low temperature plasma could be an effective tool to decrease the inflammatory response time and the inflammation was further alleviated with increased treatment time.

Highlights

Acute inflammation is a beneficial anti-injury stress reaction that can protect the human body against a wound, which is extremely important for the body’s defense system
The results showed that the number of inflammatory cells decreased from 2312.17 ± 242.52 to 880.17 ± 89.08 after plasma treatment for 3 min
Our results indicated that dielectric barrier discharge (DBD) low temperature plasma could be an effective tool to decrease the inflammatory response time and the inflammation was further alleviated with increased treatment time

Summary

Introduction

Acute inflammation is a beneficial anti-injury stress reaction that can protect the human body against a wound, which is extremely important for the body’s defense system. Once the acute inflammation lasts too long, it will cause several adverse reactions such as pain, excessive tissue damage, slow wound healing, and scar hyperplasia.. For some patients with low resistance, if active and effective measures are not taken on time, inflammatory cells will spread to the area surrounding the tissue gap or vascular system. Studies on chronic inflammation showed scitation.org/journal/adv that appropriate plasma intervention can motivate proliferation and migration of keratinization and fibroblast, induce gene expression related to wound healing, and promote wound healing.. There are few studies on the inflammatory reaction by plasma treatment in the acute stage of a wound. A large number of reactive oxygen species (ROS) produced by DBD have a good effect on killing inflammatory cells, which provides a possibility for the combination of DBD with wound treatment.. Compared with the plasma jet, dielectric barrier discharge (DBD) as a plasma source has more potential in treating different sizes of wounds. On the one hand, a large number of reactive oxygen species (ROS) produced by DBD have a good effect on killing inflammatory cells, which provides a possibility for the combination of DBD with wound treatment. On the other hand, the active substances produced by DBD enhance the absorption of extravasate and accelerate the repair of the wound.

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Conclusion