Abstract
The aim of our study was to examine the effects of 12-month therapy with recombinant growth hormone (rGH) on the blood antioxidant system in children with growth hormone deficiency (GHD). Total antioxidant capacity (TAC) of plasma was measured by FRAP (ferric reducing antioxidant power or ferric reducing ability of plasma); activities of superoxide dismutase (SOD) and catalase (CAT) in erythrocytes were assessed; non-protein thiols (NT) and ceruloplasmin (CP) levels were also measured. These parameters were determined before and after 12 month of rGH treatment. Eleven treatment-naive prepubertal children with growth hormone deficiency were included in the study. Another 11 prepubertal children comprised a control group. Before rGH treatment, TAC of plasma and NT level in the control group were significantly lower (726 ± 196 vs. 525 ± 166 µmol/L, P = 0.0182 and 0.92 ± 0.18 vs. 0.70 ± 0.22 µmol/ml, P = 0.0319, before and after the therapy, respectively). The only parameter that significantly (19.6 ± 4.7 vs. 14.5 ± 3.4 Units/g Hb, P = 0.0396) exceeded the same in the control group after rGH therapy was SOD activity. However, none of the measured parameters of antioxidant system in GHD children, except for TAC (525 ± 166 vs. 658 ± 115 µmol/L, P = 0.0205), exhibited significant improvement toward the end of the 12-month treatment period, although non-significant changes in CAT activity and CP level were also observed. This work has demonstrated that some parameters of the blood antioxidant system are out of balance and even impaired in GHD children. A 12-month treatment with rGH resulted in a partial improvement of the antioxidant system.
Highlights
Free radicals and other reactive species are thought to play an important role in many human diseases
As of today only a small number of publications are dedicated to OS in growth hormone deficiency (GHD) children, those subjected to treatment with recombinant growth hormone (rGH)
This paper demonstrated that OS parameters (index of susceptibility of low-density lipoprotein (LDL) to in vitro oxidation, malondialdehyde and vitamin E levels) in GHD children were substantially higher than those in the healthy control group, whereas after one year of rGH therapy these parameters returned to normal levels
Summary
Free radicals and other reactive species are thought to play an important role in many human diseases. We believe that some parameters of the blood antioxidant status could be used for evaluation of OS. It is important to estimate OS in GHD children. Treatment with recombinant growth hormone (rGH) and a subsequent increase in linear growth rate can lead to acceleration of metabolic processes and be accompanied by changes in the blood antioxidant status parameters. As of today only a small number of publications are dedicated to OS in GHD children, those subjected to treatment with rGH. This paper demonstrated that OS parameters (index of susceptibility of low-density lipoprotein (LDL) to in vitro oxidation, malondialdehyde and vitamin E levels) in GHD children were substantially higher than those in the healthy control group, whereas after one year of rGH therapy these parameters returned to normal levels
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