Abstract
The sialome comprises sialylated glycoproteins and glycolipids that play essential roles in cell-cell communication. Using azide-modified molecular precursors of sialic acids and copper-free click chemistry, we visualized the spatiotemporal dynamics of the sialome in live zebrafish embryos.
Highlights
All cells in vertebrates are adorned with sialylated glycoproteins and glycolipids that collectively comprise the sialome.[1,2] Because sialic acid residues often occupy terminal positions on these structures, they are well situated to control biological interactions at the cell surface
We previously found that sialylated glycans can be labeled in cultured cells and live mice by supplying the sialic acid biosynthetic pathway with azide-labeled analogues of the biosynthetic precursor N-acetylmannosamine (ManNAc).[18,19,20]
To temporally distinguish populations of sialoglycoconjugates, we performed two difluorinated cyclooctyne (DIFO) labeling reactions in succession, thereby marking sialic acids biosynthesized at different stages of development with distinct fluorophores (Figure S1 in the Supporting Information)
Summary
All cells in vertebrates are adorned with sialylated glycoproteins and glycolipids that collectively comprise the sialome.[1,2] Because sialic acid residues often occupy terminal positions on these structures, they are well situated to control biological interactions at the cell surface. We previously employed similar strategies to image mucin-type O-glycans and fucosylated glycans in zebrafish.[27,28,29] We extend the metabolic labeling approach to image sialic acids in this model organism. To label sialylated glycans in zebrafish over the first four days of development, we incubated embryos in medium containing Ac4ManNAz from 4 to 96 h post-fertilization (hpf; Figure 1 B).
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
