Abstract
Scanning Electron Microscopy (SEM), a surface imaging technique, was a late entrant to cell biology, because resolving power was too low to see details of the cell, but this changed in the mid-1980s by the introduction of field emission gun SEMs (FEGSEMs). Since then, SEM has produced a new appreciation of both the cell surface and its interior organization previously unrealized by the higher resolving, but 2D thin section transmission electron microscopy (TEM) due to the depth of field and three-dimensional (3D) surface imaging characteristics of SEM. This article summarizes the instrumentation, specimen preparation and handling, together with examples of some of the novel results produced by this technology.
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