Imaging processing settings of bright field staining images

Abstract

Abstract. Background: Clear images are vital to studying the cells and exploring the relationships between different parts of cells. Thus, histology is important to view the microscopic structures of cells by using different colors to show. However, the pipeline of getting a better and more useful image of bright field staining images is still a challenge. This article introduces different processed images to get a good condition to improve image quality. Methods: Four staining ways, including Periodic acidSchiff (PAS) staining, Hematoxylin and Eosin (H&E) staining, Trichrome Staining, and immunohistochemistry (IHC), have been used on the liver, heart, and brain sections. Photoshop and Image J were used to process images of different conditions. The software settings have been compared. Results: 60X Oil immersion can provide nice details for the liver, heart, and brain with PAS, Trichrome, and H&E staining. By comparing different settings on software, the following settings can be optimal options: 300 PPI resolution for optimal size and clarity, gamma to adjust the light and dark, color balance to make the colors more comfortable to the eye, TIFF format to meet most requirements. Image J, compared to Photoshop, can solve many image processing and analysis problems with a smaller installation package and is free to download. Conclusions: By using optimized settings of image software, researchers can get better pictures and assist in solving more scientific problems.