Abstract

Abstract The role of the illumination system of the confocal microscope, and in particular the first pinhole, is discussed. The effects of a finite sized source on the imaging performance, including the three-dimensional optical transfer function, are investigated. Two forms of source are considered, either a coherent Gaussian beam of finite size or an incoherent disc of finite size. For a confocal microscope using optical fibres as source and detector, the optimum geometry is derived for maximizing signal strength.

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