Abstract

Video microscopy and digital image processing were utilized to study physiological functions in transparent animals. The following applications will be discussed: (a) in the spider Pholcus phalangioides (1) image analysis showing interactions between heart and midgut activity and (2) imaging of the transition of blood from the closed arterial to the open venous system by injection experiments and of the structure of the open blood spaces in the legs by analysing blood cell movements; (b) image analysis of limb movements showing a lack of hypoxic ventilatory control as well as measurements of whole animal oxygen equilibrium curves in the water flea Daphnia magna; and (c) image analysis of ventilatory and circulatory convection showing a lack of adaptations to hypoxia in the larvae of the North Sea fish Agonus cataphractus.

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