Abstract
We used Raman micro-spectroscopy technique to analyze the molecular changes associated with oral squamous cell carcinoma (SCC) cells in the form of frozen tissue. Previously, Raman micro-spectroscopy technique on human tissue was mainly based on spectral analysis, but we worked on imaging of molecular structure. In this study, we evaluated the distribution of four components at the cell level (about 10 μm) to describe the changes in protein and molecular structures of protein belonging to malignant tissue. We analyzed ten oral SCC samples of five patients without special pretreatments of the use of formaldehyde. We obtained cell level images of the oral SCC cells at various components (peak at 935 cm−1: proline and valine, 1004 cm−1: phenylalanine, 1223 cm−1: nucleic acids, and 1650 cm−1: amide I). These mapping images of SCC cells showed the distribution of nucleic acids in the nuclear areas; meanwhile, proline and valine, phenylalanine, and amide I were detected in the cytoplasm areas of the SCC cells. Furthermore, the peak of amide I in the cancer area shifts to the higher wavenumber side, which indicates the α-helix component may decrease in its relative amounts of protein in the β-sheet or random coil conformation. Imaging of SCC cells with Raman micro-spectroscopy technique indicated that such a new observation of cancer cells is useful for analyzing the detailed distribution of various molecular conformation within SCC cells.
Highlights
Oral squamous cell carcinoma (SCC) is one of the most common cancers in the craniocervical area, and SCC accounts for approximately 90% of all cancers in the oral cavity [1]
In earlier reports of malignant tissue based on Raman spectroscopy, the main discussion concerns the difference in spectra between malignant and normal tissues [3,4,5,6,11,12,13,14,15,16]
Based on the present study, we can, for the first time, identify the SCC cells as a mapping image reflecting the distributions of proline and valine, phenylalanine, nucleic acids, and amide I in oral SCC cells
Summary
Oral squamous cell carcinoma (SCC) is one of the most common cancers in the craniocervical area, and SCC accounts for approximately 90% of all cancers in the oral cavity [1]. Peak Position of Amide I in the SCC and Normal Areas, and in the Nuclei and Cytoplasm of SCC Cells. The peak position of amide I is at 1648.7 cm−1 within the SCC area (Group B) and at 1645.2 cm−1 within the normal area (Group A) (Table 1). In the SCC cells, the amide I band shifted to the high frequency side of about 3.9 cm−1 in the nuclei area (Group C) compared to the cytoplasm area (Group D). Molecules 2021, 26, x FOR PEER REVIEW position between the SCC area and normal area, and in the nucleus and cytoplasm of the SCC cells. SCC sample 3–4 (n D= )2.0S0ipgoniinfitcsa) nptatdieinffte2rences (p < 0.05) were observed in the changes in the Amide I peak SCC sample 5–7 (n p=o3s0i0tiopnoinbtest)wpeaetinentht 3e SCC area and normal a1r6e4a8,.7ancmd −in the nucleus1a.4n9d cytoplasm* of the SCC cells.
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
