Abstract
We demonstrate that time-resolved second-harmonic (SH) light scattering, when applied as an imaging modality, can be used to spatially resolve the adsorption and transport rates of molecules diffusing across the membrane in a living cell. As a representative example, we measure the passive transport of the small amphiphilic ion, malachite green, across the plasma membrane in living human dermal fibroblast cells. Analysis of the time-resolved SH images reveals that membrane regions enduring higher stress exhibit slower transport rates. It is proposed that this stress-transport relation is a result of local enrichment of membrane rigidifiers as part of the cell response to maintain membrane integrity under high strain.
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