Abstract
We demonstrate imaging of blood cells enclosed in chicken skin tissue using speckle scanning microscopy (SSM). Clear images of multiple cells were obtained with subcellular resolution and good image fidelity, provided that the object dimension was smaller than the maximum scanning range of the speckle pattern. These results point to the potential and the challenges of using SSM technique for biological imaging.
Highlights
Bertolotti et al [1] recently reported a method for imaging fluorescent objects behind scattering media
Using optical speckle scanning microscopy [1], we demonstrate that clear images of multiple cells can be obtained through biological scattering tissue, with subcellular resolution and good image quality, as long as the size of the imaging target is smaller than the scanning range of the illuminating speckle pattern
We show that within these constraints speckle scanning microscopy (SSM) can be used to image blood cells enclosed in scattering biological tissues
Summary
Bertolotti et al [1] recently reported a method for imaging fluorescent objects behind scattering media. The technique is based on scanning the speckle pattern produced by the scatterers across the object and collecting the resulting fluorescence back through the scattering medium. We refer to this method as speckle scanning microscopy (SSM). SSM is one of the many methods recently reported to generate optical images in scattering media. The images we obtained were of good quality, demonstrating that SSM has a potential in biological applications
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