Imaging and quantifying Brownian motion of micro- and nanoparticles using phase-resolved Doppler variance optical coherence tomography

Chang Soo Kim,Jun Zhang,Wenjuan Qi,Zhongping Chen,Young Jik Kwon

doi:10.1117/1.jbo.18.3.030504

Chang Soo Kim, Jun Zhang + Show 3 more

Open Access

https://doi.org/10.1117/1.jbo.18.3.030504

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Abstract

Different types and sizes of micro- and nanoparticles have been synthesized and developed for numerous applications. It is crucial to characterize the particle sizes. Traditional dynamic light scattering, a predominant method used to characterize particle size, is unable to provide depth resolved information or imaging functions. Doppler variance optical coherence tomography (OCT) measures the spectral bandwidth of the Doppler frequency shift due to the Brownian motion of the particles utilizing the phase-resolved approach and can provide quantitative information about particle size. Spectral bandwidths of Doppler frequency shifts for various sized particles were quantified and were demonstrated to be inversely proportional to the diameter of the particles. The study demonstrates the phase-resolved Doppler variance spectral domain OCT technique has the potential to be used to investigate the properties of particles in highly scattering media.

Highlights

Different types and sizes of micro- and nanoparticles have been synthesized and developed for numerous applications
Low coherence interferometry (LCI) assisted dynamic light scattering (DLS) has been developed based on slow time domain and spectral analyzing method, providing limited depth range with stationary[11] or limited reference path length delay.[12,13,14]
Differences in diffusion with micro- and nanoparticles were demonstrated by employing time domain-optical coherence tomography (OCT) and spectral domain-OCT (SD-OCT) that is based on the power spectrum of the temporal fluctuations of the OCT magnitude.[19]

Summary

Introduction

Different types and sizes of micro- and nanoparticles have been synthesized and developed for numerous applications. Since the Brownian motion will broaden the spectral bandwidth of the Doppler frequency shift (Doppler bandwidth), PR-DV- OCT, which measures the Doppler bandwidth, will be affected by the Brownian motion and has a strong correlation with particle size.[23] In this study, we report on quantification of particle size with a phase-resolved Doppler variance SD-OCT system.

Results

Conclusion