Abstract
Surface-enhanced Raman spectroscopy (SERS) is a highly selective technique that can be used for imaging of single algae cells. In contrast to normal Raman spectroscopy, SERS utilizes light interaction with colloidal gold or silver particles working as antennas to match the sensitivity of fluorescence measurements. Furthermore, SERS enables a more profound picture of not only the analyte of interest but also the present biological matrix without the need for additional fluorescence labelling. The introduction of an internal standard in the form of a thiol self-assembled monolayer (SAM) on the colloidal gold or silver particles can be used to normalize the SERS response that otherwise would also depend on the locations of the colloid particles in the microscope image.In light of the vast amounts of data that is generated in each spectrum and the large variance in enhancement signal, multivariate analysis is necessary for accurate evaluation. This can be done by the use of transposed orthogonal projections to latent structures (T-OPLS), where the variations of properties in the reference spectra, Y table, and the variation in spectra, X table, are correlated.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
