Abstract
INTRODUCTIONThe understanding of neuronal circuits has been greatly advanced by the ability to simultaneously image action-potential generation within large populations of neurons. This protocol describes bulk loading of brain slices with acetoxymethyl (AM) ester calcium indicators to monitor action-potential activity in functional neuronal circuits. The imaging of calcium influx into neurons provides an indirect but accurate measure of action-potential generation within individual neurons. The key advantage of the technique is that it allows the researcher to densely sample the activity of a large population of neurons with single-cell resolution.
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