Imagens digitalizadas na avaliação do teste de tetrazólio em sementes de trigo

Abstract

The tetrazolium test can be an alternative to obtain fast results of the physiological potential of wheat seeds. In this context, the objective of the present study was to evaluate the efficiency of the tetrazolium test through the evaluation by analysis of digitized images in the determination of the physiological quality of wheat seeds.The experiment was carried out with 22 lots of wheat seeds, submitted to the determination of the physiological potential, including the tetrazolium test evaluated by means of digitized image analysis.For digitization, after longitudinal bisection, and staining in 0.075% tetrazolium solution, the seeds were grouped on tabletop scanner glass, stored and classified into four vigor classes. Data were submitted to analysis of variance, with means grouped by the Scott-Knott test, at 5% of significance; we also performed an analysis of the simple correlation coefficients between tetrazolium test results and other tests.The evaluation of the tetrazolium test through the analysis of digitized images grouped the lots in three levels of vigor.The method is effective in determining the vigor and viability of wheat seeds, because the possibility of expanding the images that allows precise analysis of the embryonic axis structures.