Abstract
Objective: The purpose of the study is to explore the potential competing endogenous RNA (ceRNA) network and investigate the molecular mechanism of lncRNA small nucleolar RNA host gene 1 (SNHG1) in hepatocellular carcinoma (HCC) development. Methods: By analyzing the data of hepatocellular carcinoma in the TCGA databases, we concluded differentially expressed lncRNA and miRNA profiles and constructed ceRNA networks related with the prognosis of HCC patients. qRT-PCR, western blotting, MTT, transwell assay and the nude mice model were employed to test the effects of SNHG1 and LMNB2 on tumor proliferation and growth in vitro and in vivo. Results: In the study, we identified 115 mRNAs, 12 lncRNAs and 37 miRNAs by intersecting differentially expressed genes (DEGs) in the TCGA and StarBase database. Then SNHG1-miR-326-LMNB2 pathway came into notice after further survival analysis and Hub gene screening. Our results showed SNHG1 expression was upregulated significantly in HCC tissues and cell lines. Downregulation of both LMNB2, the target of miR-326 in HCC, and SNHG1 inhibited tumor proliferation and growth in vitro and in vivo. Furthermore, SNHG1 could regulate LMNB2 expression through binding to miR-326 in HCC cell lines. Conclusion: SNHG1 is a promising prognostic factor in HCC and SNHG1-miR-326-LMNB2 axis may be a potential therapeutic target for HCC.
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