Abstract
We present a frequency domain analysis of the image resolution of optical tomography systems. The result of our analysis is a description of the spatially-variant resolution in optical tomographic image after reconstruction as a function of the properties of the imaging system geometry. We validate our model using optical projection tomography (OPT) measurements of fluorescent beads embedded in agarose gel. Our model correctly describes both the radial and tangential resolution of the measured images. In addition, we present a correction of the tomographic images for the spatially-varying resolution using a deconvolution algorithm. The resulting corrected tomographic reconstruction shows a homogeneous and isotropic pixel-limited resolution across the entire image. Our method is applied to OPT measurements of a zebrafish, showing improved resolution. Aside from allowing image correction and providing a resolution measure for OPT systems, our model provides a powerful tool for the design of optical tomographic systems.
Highlights
Three dimensional imaging of cells and small animals is of great importance in the biological and medical sciences as our knowledge on human diseases is based on the structure and function of cells and organisms
We present a frequency domain analysis of the image resolution of optical tomography systems
The result of our analysis is a description of the spatially-variant resolution in optical tomographic image after reconstruction as a function of the properties of the imaging system geometry
Summary
Three dimensional imaging of cells and small animals is of great importance in the biological and medical sciences as our knowledge on human diseases is based on the structure and function of cells and organisms. Light sheet microscopy uses a light illumination sheet and images the emission from perpendicular to the sheet. OPT is used for imaging small animals such as zebrafish and mouse embryos, whole organs taken from adult mice, and plant tissue. It can be used in-vivo, or ex-vivo in combination with optical clearing techniques that suppress light scattering. OPT resembles X-ray CT, in fluorescent mode, OPT is more similar to single-photon emission computed tomography (SPECT) imaging. In both cases optical radiation is used instead of X-rays or gamma quanta
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