Abstract
Background. Microscopes are omnipresent throughout the field of biological research. With microscopes one can see in detail what is going on at the cellular level in tissues. Though it is a ubiquitous tool, the limitation is that with high magnification there is a small field of view. It is often advantageous to see an entire sample at high magnification. Over the years technological advancements in optics have helped to provide solutions to this limitation of microscopes by creating the so-called dedicated “slide scanners” which can provide a “whole slide digital image.” These scanners can provide seamless, large-field-of-view, high resolution image of entire tissue section. The only disadvantage of such complete slide imaging system is its outrageous cost, thereby hindering their practical use by most laboratories, especially in developing and low resource countries. Methods. In a quest for their substitute, we tried commonly used image editing software Adobe Photoshop along with a basic image capturing device attached to a trinocular microscope to create a digital pathology slide. Results. The seamless image created using Adobe Photoshop maintained its diagnostic quality. Conclusion. With time and effort photomicrographs obtained from a basic camera-microscope set up can be combined and merged in Adobe Photoshop to create a whole slide digital image of practically usable quality at a negligible cost.
Highlights
Microscopes are omnipresent throughout the field of biological research
As technological advancements are happening at the speed of light, surveys indicate that about 50% of pathology courses already have or expect to implement virtual microscopy [2] at least in the west
We propose a near perfect virtual slide that can be definitely created provided that all guidelines are followed with interest, dedicated effort, time, and patience
Summary
Microscopes are omnipresent throughout the field of biological research. With microscopes one can see in detail what is going on at the cellular level in tissues. Over the years technological advancements in optics have helped to provide solutions to this limitation of microscopes by creating the so-called dedicated “slide scanners” which can provide a “whole slide digital image.”. These scanners can provide seamless, large-field-of-view, high resolution image of entire tissue section. With time and effort photomicrographs obtained from a basic camera-microscope set up can be combined and merged in Adobe Photoshop to create a whole slide digital image of practically usable quality at a negligible cost. Whole slide imaging (WSI) technology permits glass slides to be scanned and viewed on a computer screen This technology is referred to as virtual microscopy (VM). As technological advancements are happening at the speed of light, surveys indicate that about 50% of pathology courses already have or expect to implement virtual microscopy [2] at least in the west
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