Image-guided cell sorting using fast scanning lasers

Xinyu Chen,Yu-Hwa Lo,Rui Tang,Yuanyuan Han,Chang-Hung Lee,Weian Wang,Jiajie Chen,Sung Hwan Cho,Ian Y Lian,Alex Ce Zhang,Ivan Gagne,Yi Gu,Lauren Waller,Zunming Zhang

doi:10.1063/1.5144117

Abstract

Highlights

Classification, detection, and isolation of different cell types among cell populations can bring significant insight to biology and medicine.1,2 Cell heterogeneity in biological systems has been a mechanism of maintaining the system’s stability and functionality.3 The cell-to-cell differences in structure, composition, and morphology have demonstrated functional consequences in a variety of biological processes including cell adhesion, immune interactions, intracellular signaling, and cell growth.4–7 detecting and classifying new cell types in highly heterogeneous biological samples is crucial to understand the complex functions and behaviors of biological systems
Microscopy and fluorescence-activated cell sorters (FACS) are the workhorses to perform such functions, as the former produces high information content, i.e., imaging features of cells, and the latter has the high throughput and the ability to isolate specific cell types based on expression levels of biomarkers
To characterize the performance of the image-guided cell sorter, we first imaged suspended fluorescent (Dragon Green) polystyrene beads with a mean diameter of 0.96 μm (Bangs Laboratories, FSDG004)

Summary

Introduction

Classification, detection, and isolation of different cell types among cell populations can bring significant insight to biology and medicine. Cell heterogeneity in biological systems has been a mechanism of maintaining the system’s stability and functionality. The cell-to-cell differences in structure, composition, and morphology have demonstrated functional consequences in a variety of biological processes including cell adhesion, immune interactions, intracellular signaling, and cell growth. detecting and classifying new cell types in highly heterogeneous biological samples is crucial to understand the complex functions and behaviors of biological systems. Classification, detection, and isolation of different cell types among cell populations can bring significant insight to biology and medicine.. Detecting and classifying new cell types in highly heterogeneous biological samples is crucial to understand the complex functions and behaviors of biological systems. All of the abovementioned reasons underscore the importance of detecting, classifying, and isolating cell types even for cells of very low abundance. Microscopy and fluorescence-activated cell sorters (FACS) are the workhorses to perform such functions, as the former produces high information content, i.e., imaging features of cells, and the latter has the high throughput and the ability to isolate specific cell types based on expression levels of biomarkers. Combining microscopy and FACS, cell analysis can be coupled to the downstream genomic analysis to produce a full spectrum of information of complex biological systems

Methods

Results

Conclusion