Image changes in chlorophyll fluorescence of cucumber leaves in response to iron deficiency and resupply

Abstract

AbstractIron (Fe) is an essential element for plants and its deficiency causes decrease not only in the photosynthetic rate but also in the actual photosystem II efficiency at steady‐state photosynthesis. The aim of this work was to determine the effect of Fe deficiency in plants of Cucumis sativus (L.) in two different experimental conditions. In the first experiment, plants were grown with or without Fe for 7 d. After 7 d, Fe‐deficient plants were resupplied with Fe and sampled after 12 h and 48 h. In the second experiment, plants were grown with Fe in the nutrient solution for 3 d and after this period, Fe was withdrawn and plants sampled after 3 and 6 d. Iron and chlorophyll (Chl) concentration and Chl‐fluorescence imaging were measured. In cucumber leaves subjected to Fe deficiency, fluorescence imaging of Chl a evidenced spatial changes on leaf lamina. Following Fe deficiency both after 7 d (Exp. 1) or 6 d (Exp. 2) leaves showed a slight, nonsignificant decrease in Fv/Fm ratio. However Chl‐fluorescence parameters determined in light conditions showed significant changes which indicate an alteration in the photosynthetic process. Surprisingly, the effect of Fe deficiency was more pronounced in leaves of plant of Exp. 2 as compared to those that had grown in complete absence of Fe (Exp. 1). In the latter case down‐regulated mechanisms preserved leaves from irreversible photoinhibition leading to complete recovery when plants were resupplied with the microelement.