IM-03 CD206 expression in peripheral blood-derived induced-microglia-like cells as a surrogate biomarker for the specific immune microenvironment of glioma

Abstract

INTRODUCTION: As recent advancement of multimodal treatments including immune-check point inhibitors have not led to massive outcome improvement of glioma. Targeting the peculiar immune microenvironment of glioma is a promising approach to innovate a breakthrough treatment, however, there remains to be technical and ethical burdens for monitoring the bioactivities of immune cells in neural tissues. Herein, we examined the feasibility of non-invasive monitoring of glioma-associated microglia/macrophages (GAM) properties through utilization of our originally developed induced-microglia-like (iMG) cells technique. METHODS: We isolated primary microglia (pMG) from surgically-obtained brain tissues of 15 patients with neurosurgical diseases. We induced iMG cells from monocyte extracted from their corresponding peripheral blood by MACS treatment. Expression profiles of representative markers for an M1 and M2 microglia phenotype were analyzed in both pMG and iMG cells by qPCR. RESULTS: q-PCR revealed that a significant correlation of expression level of microglial markers between pMG and the corresponding iMG in each patient. Synchronous upregulations of CD206 were exclusively detected in patients with glioma. CONCLUSION: The present study suggested that iMG cells can be a less-invasive monitor tool for disease-related bioactivity of microglia, thereby seem to be utilized as an intermedium for investigation of relationship between microglia and neuronal diseases including glioma. CD206 upregulation detected by iMG technique can surrogate the specific microenvironment of glioma surrounding tissues and might be utilized as a future biomarker of glioma.