Abstract
Molecular motors of the kinesin and dynein superfamilies are the driving force behind intracellular transport, cell division and cell propulsion. Inside the chemosensory cilia of Caenorhabditis elegans two kinesin-2-family motors, kinesin-II and OSM-3-kinesin, cooperate to build and maintain the cilium, in a process called intraflagellar transport (IFT). In order to quantitatively asses IFT-kinesin function at endogenous expression levels, we have generated transgenic worms using Mos1-mediated single-copy integration of transgenes encoding fluorescently-labeled-IFT kinesins. Ultrasensitive wide-field and confocal fluorescence microscopy allows accurate mapping, counting, tracking and correlation of these molecular machines inside living, multicellular organisms. This approach allows unprecedented insight into IFT and motor-driven processes in general.
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