Abstract
Escherichia coli B/r exposed to visible light, oxygen and Rose Bengal was killed by singlet oxygen. There was no evidence of cytotoxicity when the organisms were suspended in non-illuminated dye (2 × 10-4 mol/1). Incubation in oxygenated dye before exposure to light induced a quicker onset of exponential death but the eventual rate was slower than that of organisms exposed continuously to light. The concentration of intracellular adenosine triphosphate (ATP) of illuminated E. coli B/r declined before there was a demonstrable reduction in the number of viable organisms. A carotenoid containing species of Rhodotorula was not killed by the irradiated dye even with a photoflux greater than that used for the coliform. There was no diminution in the ATP content of this yeast with illumination. Thus this study has shown that ATP photometry can be used to explore the kinetics of photoinactivation of sensitive micro-organisms.
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