Abstract
The aim of this study was to use metagenomics to investigate how Illite/smectite clay (I/S) affected Auxiliary Activities (AA1, AA2, AA3) thereby enhancing lignin decomposition and humification. Metagenomics analysis illustrated that the abundances of AA1, AA2, AA3 in test group (TG) with 10% I/S were 28.98%, 15.18%, 14.36% higher than that in reference group (RG), respectively. Meanwhile, I/S greatly boosted the efficiency of lignin degradation (17.96%) and humus formation (7.16%) compared with RG (13.10%, 3.49%). Furthermore, Actinobacteria was the microorganism with the greatest contribution in RG and TG to secreting AA1 (41.12%, 57.37%), AA2 (62.42%, 65.28%), AA3 (47.04%, 55.47%). Redundancy analysis (RDA) demonstrated that I/S could make the laccase encoding gene-AA1 contribute more to HS formation relative to AA2 and AA3. In conclusion, applying I/S in cattle manure composting effectively improved the abundance, bioavailability of lignin degradation functional gene enzymes and the composting efficiency.
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