마테(Ilex paraguariensis) 추출물/분획물의 항산화능 및 타이로시네이즈 저해 활성

Abstract

In the present study, 50% ethanol extract, the ethyl acetate and aglycone fraction were prepared from mate (Ilex paraguariensis) and their antioxidative ability was evaluated. The yields of extract and fractions were 32.0, 4.48 and 0.82% per dried powder, respectively. Free radical scavenging activities were performed by 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay and total antioxidant capacity was estimated using luminol-dependent chemiluminescence assay. Free radical scavenging activities () of 50 % ethanol extract, ethyl acetate fraction and aglycone fraction were 8.83, 5.84 and , respectively. Their total antioxidant capacities () were similar to that of L-ascorbic acid (), known as a prominent water soluble antioxidant, in all extracts and 50% ethanol extract () was the most effective. The cellular protective effects on the -induced cellular damage of erythrocytes were evaluated and the results showed that all extracts were significantly higher than (+)--tocopherol at . Especially, the value of aglycone fraction was 5 times higher than (+)--tocopherol at and . The inhibitory effects of the ethyl acetate and aglycone fractions on tyrosinase were similar to arbutin, known as the whitening agent in cosmetics. These results suggest that the extracts of mate have the applicability as antioxidant and anti-aging cosmeceutical ingredients.