Ileorectal anastomosis as a model for digestion studies in sheep: effect on water, acid-base, electrolyte and energy balance of the whole animal, as well as on the anatomy of the digestive tract

Abstract

ABSTRACTWater balance, electrolyte and acid-base status of a group of five South African Mutton Merino wethers given chopped lucerne hay ad libitum was determined before and after ileorectal anastomosis. In general, the sheep recovered rapidly from surgery, resuming, within 10 days, a level of dry matter intake only slightly less than pre-operative levels (1323 (s.e. 147) and 1419 (s.e. 196) g/day respectively, P > 0·05). A new water balance was established at about the same time, when sheep with an ileorectal anastomosis drank about 2500 ml/day more water, while losing about 2400 ml/day more water in the faeces and about 365 ml/day less water in the urine than did the control sheep. Most of the electrolyte and acid-base parameters tested were not significantly affected by the anastomosis but the concentrations of oxygen and potassium in the blood of sheep with an anastomosis were significantly lower (P < 0·001), while those of chloride (P < 0·001) and bicarbonate (P < 0·05) were significantly higher, than before surgery. These observations, together with normal blood pH values, suggest that bypassing the large intestine caused a compensated metabolic alkalosis.Energy balance was determined on the five sheep with an ileorectal anastomosis as well as five control animals in an indirect, open-circuit calorimeter. Despite a significantly lower (P < 0·05) loss of nitrogen and energy via the urine (due to a lower output of urine) in sheep with an ileorectal anastomosis, total heat loss, whether absolute or relative to energy intake, did not differ from control values and therefore indicated that basal metabolic rate was not significantly affected.From post-mortem examinations, anastomoses appear to have induced a marked atrophy of the bypassed caecum and colon, whilst the mass of the large intestine was significantly less than in control animals, despite the significant enlargement of the rectum. On the other hand, the small intestine appeared unaffected, either macroscopically or histologically.The most significant physiological changes that occurred on bypassing the large intestine were related to loss of water reabsorbtion. However, good adaptation to the increased turn-over of water was evident, providing free access to water and a salt lick was allowed.