Abstract
ILC3-derived acetylcholine promotes protease-driven allergic lung pathology
Highlights
Leffler J, Read JF, Jones AC, Mok D, Hollams EM, Laing IA, et al Progressive increase of FcepsilonRI expression across several PBMC subsets is associated with atopy and atopic asthma within school-aged children
We have identified association of ILC3s with protease-induced lung pathology following acute papain challenge of wild-type (WT) C57BL/6 mice
Lin–CD451CD1271ICOS1 ILC2s), we identified an increase in the choline acetyltransferase (ChAT)-expressing ILC3-enriched
Summary
CD451CD3–Lin– cells did show IL-17 production following phorbol myristate acetate restimulation. Airway cellular infiltration and detection of pulmonary IL-13 and IL-17A by ELISA is shown. Airway resistance to methacholine challenge following papain challenge in Rorc–/– mice. Detection of pulmonary IL-13 and IL-17A by ELISA in papain-challenged Rorc–/– mice. Detection of ILC2s (Lin–CD451CD1271ICOS1) and NCR1 ILC3s (Lin–CD451CD1271ICOS–NKp461) in the lung by flow cytometry. As expected, detection of ILC3 subsets such as NCR1 ILC3s (Lin–CD451CD1271ICOS–NKp461) in Rorc–/–. This body of work identifies a previously unappreciated, T-cell–independent role for
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