Abstract
Background: Interleukin-27 (IL-27) may contribute to steroid-resistant asthma, however, the mechanisms mediating these effects and association of IL-27 with asthma endotypes are unclear. AIM: The study aims to examine sputum IL-27 mRNA and protein levels in asthma endotypes and inflammation. Methods: Induced sputum was collected from 80 adults with stable asthma; 30 neutrophilic asthma (NA), 30 eosinophilic asthma (EA) and 20 paucigranulocytic asthma (PGA). Total and differential cell counts, mRNA and protein expression of IL-27 and TNF-α were assessed. Results: Patients had a mean age of 55 years and 59% were female. Gene expression for IL-27EBI3 and TNF-α were increased in NA compared with EA and PGA (p 1 % predicted (r=-0.28, p=0.04). No significant correlation existed between mRNA and protein levels for IL-27 or TNF-α (p>0.05). Conclusion: Lack of any correlation between IL-27R, IL-27p28 and IL-27EBI3 mRNA with IL-27 protein suggests differential translation of IL-27 in asthma endotypes. Association between eosinophil% and IL-27 protein suggests a functional role of IL-27 in EA.
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