Abstract
Objective: We studied the relationship between the severity of inflammation and IL-1βproduction and relative expression level of IL-1βmRNA in irrigation fluid and synovial tissue obtained from the knee joint during the acute stage of a murine model of type II collagen antibody-induced arthritis (CAIA). This model is used to identify potential therapeutic markers for treating rheumatoid arthritis.Methods: Irrigation fluid and synovium tissue were harvested from the knee joint of BALB/c mice in acute stage of CAIA induction. The IL-1βprotein level was measured by enzyme-linked immunosorbent assay, and the relative expression level of IL-1βmRNA was analyzed by reverse transcription-polymerase chain reaction. Two investigators analyzed expression levels and histopathological changes.Results: IL-1βconcentration was higher in irrigation fluid from the knee joint than in the serum in the acute stage of CAIA. The relative expression level of IL-1βmRNA was elevated in synovial tissue. Histopathological changes in the knee joint and foot indicated similar severity.Conclusions: IL-1βconcentration in irrigation fluid and relative expression level of IL-1βmRNA in the synovium have potential as therapeutic markers in studying and treating CAIA.
Highlights
Rheumatoid arthritis (RA) is a chronic autoimmune disease affecting primarily the synovial tissue in many joints
Collagen typeII (CII) antibodyinduced arthritis (CAIA) is commonly used as a model of RA for screening of antirheumatic drugs because it is similar to human RA [5]
Our aim is to develop a therapeutic reference in a single knee joint using the CAIA model to simplify and the lower the cost of research on RA because we can compare therapeutic results between the knee joints in individual mice
Summary
Rheumatoid arthritis (RA) is a chronic autoimmune disease affecting primarily the synovial tissue in many joints. Anti-type II collagen antibody is an autoantibody that appears in the blood of patients with RA [3]. The collagen-induced arthritis (CIA) model is the most commonly used animal model of RA. II (CII) is a major constituent of the articular cartilage matrix proteins, and immunization with native CII in adjuvant induces autoimmune polyarthritis by stimulating the cross-reactive immune response to homologous collagen [4]. The generation of CII-specific antibodies is required for progression of CIA. CII antibodyinduced arthritis (CAIA) is a kind of murine RA model that can be induced by passive transfer of antibody to CII [5] and is used to evaluate therapeutic effects in inflammatory arthritis [6]
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