台灣人種之IL-17A、CD14、MMP-1和TLR4單一核苷酸多型性與侵犯性牙周炎之間關聯性

Abstract

The aim of the present study was to investigate the association of single nucleotide polymorphism (SNP) with aggressive periodontitis (AgP) in Taiwanese. All subjects were Taiwanese ethnicity. The design of study was a case-control study, including the test groups of aggressive periodontitis (AgP) and chronic periodontitis (CP) volunteers, and the control group of periodontitis-free healthy participants. SNPs of interleukin-17A (IL-17A), cluster of differentiation (CD14), matrix metalloproteinase-1 (MMP-1) and toll-like receptor 4 (TLR4) were chosen. According to the principle of polymerase chain reaction (PCR), real-time polymerase chain reaction (RT-PCR) was utilized to amplify the small quantity of DNA from the peripheral blood sample. The effect of Real-time was achieved to determine the expression of the four SNPs quantitatively by using fluorescence signal detection system. We used the chi-square test or Fisher’s exact test to compare the means and proportions in allele/genotype frequencies of the three groups. Logistic regression analysis was performed to measure the relationship between the genotypes and periodontitis. Analysis of the polymorphisms showed patients with periodontitis (AgP and CP) had a higher distribution of G allele (AgP:39.3%, CP:38%, H:6.8%) for CD14 SNPs, patients with AgP had higher 1G/1G genotype and 1G allele (AgP:66.1%, CP:40%, H:34.1%) for MMP-1 SNPs (p<0.05). As for IL-17A and TLR4 SNPs, we found no correlation with periodontitis. The subjects with the combined allele associated with AgP had a higher percentage than the subjects with the single allele in AgP group. These data suggest that patients with CD14 polymorphism were associated with the occurrence of AgP and CP in Taiwanese ethnicity population. The MMP-1 polymorphism (1G allele) is genetically associated with vulnerability of patients with AgP. In addition, combined occurrence of the CD14 and MMP-1 SNPs allele may raise the susceptibility of one individual to AgP.