Abstract
Use of mesenchymal stem cells from bone marrow has gained significant popularity. The iliac crest has been determined to be an effective site for harvesting mesenchymal stem cells. Review of the literature revealed that multiple techniques are used to harvest bone marrow aspirate from the iliac crest, but the descriptions are based on the experience of various authors as opposed to studied anatomy. A safe, reliable, and reproducible method for aspiration has yet to be studied and described. Connective tissue progenitors (CTPs) from native bone marrow (BM) or their culture-expanded progeny, often referred to as mesenchymal stem/stromal cells, represent a promising strategy for treatment of cartilage injuries and other problems of bone consolidation. However, the cartilage regeneration capacity of these cells remains unpredictable because of cell heterogeneity. Our experience concerning the aspiration of mesenchymal stem cells from the iliac crest and their use in patients suffering from chondropathy has given good results, with the use of the sepax2 method for processing and preparation of mesenchymal stem cells extracted from the iliac crest.
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