Abstract
Background/aim: Distinct mucosal immunoregulatory cytokine patterns are observed during the evolutive stages of CD (1). 1L-8 upregulation is associated with an increased neutrophilic infiltrate in chronic colonic lesions of patients with CD. The aim of this study was to compare IL-8 mRNA levels in the colonic and ileal mucosa of patients with CD during the different clinical stages of the disease. Methods: 18 patients with CD who had ileocolonic resection with anastomosis were studied. No patient received immunosuppressive or steroid therapy during the study. Biopsies were obtained during surgery from the healthy ileal and colonic mucosa in 18/18 patients and from chronic ileal lesions in 15/18 patients. Endoscopic ileal biopsies were also taken from early recurrent ileal lesions (scored i3 or i4 according to Rutgeerts criteria) occurring three months after surgery in 9 patients. IL-8 mRNA levels were quantified by competitive PCR and expressed as the mean number of eDNA molecules of IL-8 per molecule of [3-actin. Results:
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