Abstract
This study aimed to investigate the association of plasma TNF-α, IL-6, and lL-10 levels and cytokine gene polymorphisms [TNF-α (-308 G→A), IL-6 (-174 C→G) and IL-10 (-1082 A→G, -819 T→C and -592 A→C)] in type 2 diabetes mellitus (T2DM) and obese patients. One hundred and two T2DM patients and 62 controls were included in this study. Cytokine plasma levels were measured by the Cytometric Bead Array method. Genotyping was carried out by the polymerase chain reaction. IL-6 levels were significantly different between T2DM patients and controls. Interestingly, IL-6 levels were higher in T2DM patients with BMI > 30 kg/m2 compared with other patients and obese controls. The genotype and allele frequencies were similar between patients and controls. In the T2DM group, the SNP IL-10 -819 T/C showed a difference between the cytokine level and genotypes: IL-10 level in the TT genotype was significantly higher when compared to CC genotype. These results suggest an association between IL-6 levels and obesity, and IL-10 levels and the SNP -819 T/C in T2DM. Knowledge of these variants in T2DM might contribute to a better understanding of the role of inflammation in the etiology and progression of this disease.
Highlights
Type 2 diabetes mellitus (T2DM) is a heterogeneous group of metabolic disorders characterized by chronic hyperglycemia and represents a significant global health problem [1]
Higher IL-6 levels were found in type 2 diabetes mellitus (T2DM) patients and our results suggest that obesity acts synergistically with T2DM by modulating the increase of this cytokine
When analyzing cytokine levels in two groups, IL-6 levels were higher in the diabetic patients (p = 0.001), and no significant differences were observed for tumor necrosis factor-α (TNF-α) (p = 0.332) and IL-10 (p = 0.317)
Summary
Type 2 diabetes mellitus (T2DM) is a heterogeneous group of metabolic disorders characterized by chronic hyperglycemia and represents a significant global health problem [1]. The pathogenesis of insulin resistance and T2DM has been associated with a subclinical chronic inflammation and activation of the immune system; what triggers this inflammation is still unclear [3,4]. (IL-6), C reactive protein (CRP), plasminogen activator inhibitor-1 (PAI-1), tumor necrosis factor-α (TNF-α), vascular cell adhesion molecule-1 (VCAM-1), and intercellular adhesion molecule-1 (ICAM-1) [5,6,7,8,9,10]. Adipose tissue in an obese individual is characterized by the presence of pro-inflammatory immune cells
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
