IL-17A rs2275913 gene polymorphism in patients with diabetic nephropathy

Abstract

Introduction: Diabetic nephropathy (DN) is a major microvascular complication of diabetes, leading to end-stage renal disease (ESRD). Objectives: The aim of this study was to investigate the association between the rs2275913 polymorphism of interleukin-17 (IL-17) gene and the incidence of kidney disease in patients with type 2 diabetes (T2D). Patients and Methods: Blood samples were collected from 113 T2D patients including 56 patients with nephropathy and 57 patients without nephropathy. In addition, 150 healthy individuals were included in this study to compare the results. Gene study was conducted by restriction fragment length polymorphism-polymerase chain reaction (RFLP-PCR) method. Results: The frequency of A allele of IL-17A (rs2275913) gene polymorphism was significantly higher in patients with DN compared to healthy controls (P=0.043). In addition, serum creatinine levels were significantly higher in DN patients regardless of their genotypes (P<0.001). Conclusion: Our results showed that diabetic patients who carry at least one A allele of IL-17A (rs2275913) gene polymorphism may develop DN.