IL-17 and IL-22 induced IL-1B, IL-8, TNF-A secretion via inhibitor of nuclear factor KB kinase-A (IKKA) expression in HaCaT cell line

Abstract

The pathogenesis of psoriasis may involve the IL-23 and Th17-mediated immune responses. Th17 cells secret IL-17 and IL-22 which mediates dermal inflammation and acanthosis. As IKKAhas been previously identified as a primary regulator of keratinocyte differentiation and proliferation, we proposed that IL-17 and IL-22 might affect keratinocyte differentiation by changing the expression of IKKA. We employed HaCaT cells maintained culture medium at a low calcium concentration (0.06mM) and induced differentiation by switching high concentration (2.8mM) media with IL-17 or IL-22, then compared IKKA expression and the cell cycle.